【PEP】Senior High Biology Elective Compulsory Book 3

This course, based on the high school biology elective compulsory textbook 3, systematically explains the core areas of modern biotechnology. The content covers the fundamental theories of fermentation engineering, cell engineering, and genetic engineering, along with their practical applications, and discusses the safety and ethical issues of biotechnology. This textbook incorporates 'Inquiry & Practice' sections, aiming to cultivate students' experimental skills and scientific thinking.

Explore the mysteries of life engineering, from traditional fermentation to the cutting-edge wisdom of gene editing.

Author: Zhu Zhengwei, Zhao Zhanliang

Acknowledgments: Reviewed and approved by the National Textbook Committee Experts in 2019

1. Elucidate principles: Be able to explain the production principles and reaction equations of traditional fermented foods (such as pickled vegetables, fruit wine, fruit vinegar).
2. Master techniques: Proficiently describe the key operational points of aseptic technique for microbial culture, the streak plate method, and the spread plate method.
3. Analyze engineering: Be able to identify the basic steps of fermentation engineering and analyze its differences from traditional fermentation techniques in strain selection, process control, and product extraction.
4. Elucidate the technical principles of plant tissue culture and somatic cell hybridization, citing examples of plant cell engineering applications in agriculture and industry.
5. Explain the process and conditions of animal cell culture and the application value of stem cells, describe animal somatic cell nuclear transfer technology and the significance of cloned animals.
6. Briefly describe the physiological basis of fertilization, understand the physiological basis of embryo transfer, and the principles and key operational points of embryo splitting technology.
7. Be able to briefly describe the functions of restriction endonucleases and DNA ligases and their roles in recombinant DNA technology.
8. Be able to summarize the components of a gene expression vector and explain the function of each part.
9. Be able to describe the basic principles and process of PCR technology and understand its application in obtaining target genes.
10. Be able to rationally view the safety controversies surrounding genetically modified products and explain China's management policies and labeling system for genetic modification technology.